Research & Instrumentation

RESEARCH TOPICS

(a) Development and Validation of Bioanalytical Methods

  •  multidimensional liquid chromatography coupled to ESI (Orbitrap or QExactive) MS, MALDI TOF/TOF MS,  or ESI/MALDI ion mobility MS

                -  top-down and bottom-up proteomics

                -  isobaric labeling, metal labeling, label free quantification, bioinformatic methods

                -  coupling of molecular (MALDI/ESI) and elemental (ICP) MS; LC-MALDI MS

  •  methods for the analytics of posttranslational modifications

                -  phosphorylation, proteolytic processing (SFB 877), disulfide bonds, oxidation, glycosylation, ubiquitinylation

                -  fragmentation behavior in mass spectrometry (PSD, CID/HCD, ETD), enrichment techniques

  • methods for the characterization of bioactive peptides and for peptidomics 

  • novel matrices for MALDI MS: ionic liquid matrices

     

(b) Applications on Biological, Biotechnological and Biomedical Problems

  •  antimicrobial peptides

  •  paleo-proteomics

 

Instrumentation

ESI lc_maldi solvent_rack

  • ESI Orbitrap Velos with ETD (Thermo)

  • ESI QExactive Plus (Thermo)

  • ESI/MALDI Synapt G2S, with ion mobility (Waters)

  • 5800 MALDI TOF/TOF MS (ABSciex)

  • ESI 3000- Triple Quad (ABSciex)

  • nano-LC (U3000) (Dionex)

  • UPLC- 3000 RSLC (Dionex)

  • Analytical LC System

  • Probot for LC-MALDI MS

  • in-house bioinformatics (Raid, NAS)